Composite

Part:BBa_K2156103

Designed by: Mikhail Lujan and Carolina Sofia Gonzalez   Group: iGEM16_ColegioFDR_Peru   (2016-09-22)


LuxABGCDE With Promoter and RBS

The part is the complete combination of the Lux's A, B, G, C, D, E. This construct is meant to produce Bioluminescence, with the LuxAB beginning the structure, LuxG removing energy pathways to make the reaction more efficient, and LuxCDE synthesizing necessary substrates for the reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3824
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4915
    Illegal BsaI.rc site found at 4160
    Illegal SapI site found at 1085
    Illegal SapI.rc site found at 5796


[edit]
Categories
Parameters
None